The usual dosage range for L-Asparaginase is 50 to 200 KU/kg bodyweight daily or every alternate day, given intravenously or intramuscularly.

Test Dose : Before treatment is started a test dose of 1 to 10 KU of L-Asparaginase in 0.1 ml of distilled water should be injected subcutaneously and the injection site observed for several hours of evidence of primary hypersensitivity.